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Oral EcologiX: Your New Clinical Tool


Oral EcologiX™ | Host-Microbiome Analysis

The oral cavity is a complex ecosystem, comprising several habitats including the teeth, gums, tongue and tonsils, all colonised by bacteria. The oral microbiota is comprised of approximately 600 taxa at the species level, with different groups and subsets inhabiting different niches.

The microbiota of the oral cavity exists as a complex biofilm that remains stable despite environmental changes. However, dysbiosis, in form of infection, injury, dietary changes and risk-associated factors (e.g. smoking) may disrupt the biofilm community, favouring colonisation and invasion of pathogens. Disruption of the biofilm community to a pathogenic profile induces host immune responses, chronic inflammation and ultimately the development of local and systemic diseases.

Much of this damage is reversible if pathogenic communities are removed, and homeostasis is restored; therefore, analysis and monitoring of the oral microbiota is important for health and can be used to inform treatment plans and lifestyle factors.

Tooth Dysbiosis
Figure 1: Development of disease in the oral cavity. Oral health involves colonisation of the enamel and gingival crevice by commensal Streptococci and bacteria. Dysbiosis involving increased intake of carbohydrates, infection or injury facilitate colonisation of caries-associated and acid-producing bacteria including Streptococcus mutans, causing destruction of the enamel. Dysbiosis may also lead to microbial invasion of the mucosa and further development of subgingival biofilm, involving colonisation by “red complex” pathogens including P. gingivalis and T. denticola, leading to inflammation and destruction of tooth-supporting tissues. Figure adapted from Lamont et al. (2018).

 

The Oral EcologiX™ Profile

Methodology:
The profile uses two technologies: quantitative real-time PCR is (q-RT-PCR) is used to quantify the abundance of microbial genes. Enzyme-linked immunosorbent assays (ELISA) are used for the quantification of host proteins.

For microbiota composition analysis, the technology detects:

  • Abundance of commensal bacteria
  • Abundance of caries-associated bacteria
  • Abundance of periodontitis-associated bacteria (orange and red-complex bacteria/pathogens)
  • Presence of viral pathogen herpes simplex virus 1 (HSV-1)
  • Presence of human papillomavirus-16 genotype (HPV-16)

For host biomarker analysis, the technology detects:

  • IL-1β (pro-inflammatory cytokine)
Dependent on microbiota composition and host biomarkers, the profile will report three different states:
  • Healthy oral microbiome (homeostasis of host and microbiome)
  • Oral dysbiosis (imbalance of the oral microbiota and/or host immune response with an understanding of pathogenicity dependent on abundance of middle and/or late colonisers)
  • Oral pathogen detected (‘red complex’ species, HSV-1, HPV-16)

Turnaround Time
10 working days

 

 


Materials & Support

Oral EcologiX Sample Report

Downloads:
Sample report
Interpretive guide
Clinical matrix
– Clinical considerations (practitioner only)

Consultations:
We offer complimentary consults with our clinical education team. You can book these from your account dashboard.

Coming soon:
Case study webinars


Introductory Webinar

Sue Camp, MSc ran a webinar introducing Oral EcologiX. In 30 minutes you will learn about the test, its clinical utility, how the test is taken and how to read a report.
→ watch

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